Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose

[bookbestseller]

Sören Stark, "Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose"
English | ASIN : B09W4QJN5K | 2022 | 120 pages | PDF | 3 MB
Cis,cis-muconic acid receives increasing interest to be produced from renewables. Catabolic microbial pathways can be tailored to accumulate cis,cis-muconic acid from a range of aromatic compounds. A renewable, sustainable and under-valued resource for aromatics is lignin. In this work, using hydrothermal conversion, lignin was depolymerized into hydrolysates with up to 615 mM aromatic monomer content. Catechol-rich hydrolysates were generated for bioconversion with the previously developed cis,cis-muconic acid producers P. putida MA-9 and C. glutamicum MA-2, whereas hydrolysates were guaiacol-rich for Amycolatopsis sp. MA-2. When grown with glucose as a co-substrate, C. glutamicum MA-2 yielded 2.6 g L⁻¹ (100 % yield) cis,cis-muconic acid from catechol. Towards an even more sustainable process, glucose was then replaced by hemicellulose, a non-food renewable. Hemicellulose, a co-constituent of lignin in lignocellulose, was hydrothermally converted into a mixture of C₅ and C₆ sugars. As hemicellulose was mainly converted into xylose (91 % yield), C. glutamicum MA-2 was engineered to utilize this pentose. Fed-batch bioconversion on a catechol-rich Kraft lignin hydrolysate as well as a hemicellulose hydrolysate using C. glutamicum MA-4 yielded 4 g L⁻¹ muconic acids. As the developed process was non-competitive to feed and food, it is a promising starting point for future application in bio-based industrial settings.

Read more

RapidGator
https://rg.to/file/565564810c5f65741c8472b6bc3e2949/lfti3.7z.html
TakeFile
https://takefile.link/h04sw3rieymq/lfti3.7z.html
Fileaxa
https://fileaxa.com/dygwtdes3f9t/lfti3.7z
Fikper
https://fikper.com/u7XoKwMci3/lfti3.7z.html